Mississippi National is a 36-hole facility that is well-known for some dramatic elevation changes. Established in 1986, the Lowlands 18 is traditional style with rolling fairways and large greens playing against the bluffs.

Nine holes of the Highlands opened in 1990 with the other nine completed in 1999, and features views from high above the Hiawatha Valley into the Mississippi River bottoms and surrounding valleys. Most golfers consider the 17th as the signature hole, featuring a 150-foot drop from tee-to-green.

Mississippi National has hosted the PGA of America's Minnesota Section championship, as well as USGA qualifying for the U.S. Senior Open and the U.S. Amateur.